Ricerc@Sapienza

The ARP facility allows to measure rapid kinetic processes (micro- to milliseconds) on purified macromolecules in vitro by different spectroscopies (absorbance, fluorescence). An array of instruments are available, including stopped-flow instruments (msec-time frame ≥ 10 ms) to study folding/unfolding processes, enzyme reaction mechanisms, rapid binding or dissociation processes (absorbance and fluorescence). For faster measurements, a Hi-Tech PTJ-64 capacitor-discharge T-jump apparatus ( ≥ 20 μs) for fluorescence and absorbance experiments is available, as well as an in-house built continuous flow (original prototype) based on a capillary mixing methodology (dead-time 50 μs). An array of instruments are available, including stopped-flow instruments (msec-time frame ≥ 10 ms) to study folding/unfolding processes, enzyme reaction mechanisms, rapid binding or dissociation processes (absorbance and fluorescence). For faster measurements, a Hi-Tech PTJ-64 capacitor-discharge T-jump apparatus ( ≥ 20 μs) for fluorescence and absorbance experiments is available, as well as an in-house built continuous flow (original prototype) based on a capillary mixing methodology (dead-time 50 μs).