Ricerc@Sapienza

This study was performed to evaluate the metabolite recovery from different extraction
methods applied to Thymus algeriensis aerial parts. A high-performance liquid chromatographic
method using photodiode array detector with gradient elution has been developed and validated
for the simultaneous estimation of different phenolic compounds in the extracts and in their
corresponding purified fractions. The experimental results show that microwave-assisted aqueous
extraction for 15 min at 100 C gave the most phenolics-enriched extract, reducing extraction
time without degradation effects on bioactives. Sixteen compounds were identified in this extract,
11 phenolic compounds and five flavonoids, all known for their biological activities. Color analysis
and determination of chlorophylls and carotenoids implemented the knowledge of the chemical
profile of this plant.