Difference mid-IR nanospectroscopy on individual patches of purple membranes: The proton pump activity of bacteriorhodopsin at the nanoscale
04 Pubblicazione in atti di convegno
Giliberti V., Polito R., Ritter E., Broser M., Hegemann P., Puskar L., Schade U., Zanetti-Polzi L., Daidone I., Corni S., Rusconi F., Biagioni P., Baldassarre L., Ortolani M.
ISSN: 2162-2027
Conformational changes of the light-sensitive membrane receptor and proton pump bacteriorhodopsin is investigated by tip-enhanced mid-infrared nanospectroscopy on individual cell membrane patches, with a dramatic increase of sensitivity (in terms of number of probed molecules) compared with standard mid-infrared spectroscopy.