RNA/DNA amplification methods for the detection of bacteria and virus through an optoelectronic lab-on-chip
04 Pubblicazione in atti di convegno
Costantini F., Lovecchio N., Iannascoli L., Scala V., Faggioli F., Pucci N., Loreti S., deCesare G., Nascetti A., Caputo D.
This paper focuses on the detection of bacteria and virus achieved by means of a miniaturize portable lab-on-chip (LoC) able to perform RNA/DNA amplification. The LoC is constituted by homemade microfluidic chips which are optically and thermally coupled to an optoelectronic (OP) thin film platform. OP device combines on a single glass substrate thin film heater, sensors for temperature control, and photosensors for fluorescence detection. The LoC was tested for performing loop mediated isothermal amplification (LAMP) for the detection of the bacteria Xylella fastidiosa subsp. pauca (Xfp) and real-time reverse transcription polymerase chain reaction (RT-qPCR) of watermelon mosaic virus, respectively.