Validation of a small-size pooling approach targeting hospital surveillance of SARS-CoV-2 infection
01 Pubblicazione su rivista
Petrucca Andrea, Borro Marina, Lionetto Luana, Gentile Giovanna, Alari Antonella, Simmaco Maurizio, Santino Iolanda
DOI: 10.1017/ice.2020.380
ISSN: 0899-823X
Recent studies describing the detection of SARS-CoV-2 RNA in pools of 5 to 32 samples reported false negative rates up to 10% for large groups, suggesting that smaller
sample pools are a good compromise to increase sample processing capacity while maintaining test reliability. Since 5-sample pools were shown to efficiently detect SARS-CoV-2 RNA in RT-PCR
assays, we chose to test and validate this approach using a highthroughput RNA extraction and amplification platform.