Ricerc@Sapienza

Our research focuses on unraveling diverse aspects of extracellular vesicle (EV)-mediated intercellular communication. This includes the discovery of novel EV-like communication systems, as well as in-depth investigation into the role of lipid cargo in EV biogenesis and function, combining innovative methodologies and high-resolution microscopies. In collaboration with the University of Lyon, we revealed that lipid droplets (LDs) can be secreted by lipid-associated macrophages (LAMs), potentially via CD81-enriched membrane domains, and contribute to cell-to-cell signaling. We aim to define the mechanisms of LD secretion and assess whether this represents a conserved communication pathway. Equally important is our focus on the role of lipid-associated EVs in glioblastoma (GBM) biology, particularly the crosstalk between tumor and glial cells during chemotherapeutic treatment. Through integrated omics approaches and comprehensive functional analyses, we aim to elucidate how EVs and their molecular cargo influence GBM growth, malignancy, and progression, especially by modulating immune responses. Among our group’s activities is the use of EVs derived from waste products for the targeted delivery of drugs or bioactive molecules. Leveraging our solid expertise in the field of nanoformulations, we adapt existing approaches or develop innovative methods for isolating and loading EVs from biological waste matrices, such as bovine milk whey, to produce eco-friendly nanocarriers. As a key component in the development of new nanocarriers, our scientific interest also includes investigating the efficiency, targeting capability, bioavailability, and cytotoxicity of these nanostructured systems, using both in vitro and in vivo models.