The early work of Botta group was devoted to the structural elucidation and synthesis of biological active molecules derived from plant in vivo. Since 1984, his group have been working in the field of in vitro plant tissue cultures. The general aim of the investigation was directed toward the understanding of biosynthetic pathways of the isolated metabolites and the isolation of the responsible enzymes. During this research, new plant enzymes (e.g. peroxidases) have been isolated, purified, and characterized by chemical and kinetic studies.
