large scale purification | Ricerc@Sapienza

HPLC semi-preparativo serie LC-4000 JASCO_PREPNAT

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The required instrument is a High-Performance Liquid Chromatography system developed to operate in both analytical and preparative modes, in ultra-fast, fast, and conventional chromatography, and using columns with a particle size of 1.8 microns. The chromatographic system consists of several units and accessories that compose a versatile and high-performance instrument for the qualitative and quantitative analysis of complex mixtures and for the purification of large amounts of samples.

Application of crossflow ultrafiltration for scaling up the purification of a recombinant ferritin

Ferritin proteins are taking center stage as smart nanocarriers for drug delivery due to their hollow cage-like structures and their unique 24-meric assembly. Among all ferritins, the chimeric Archaeoglobus ferritin (HumFt) is able assemble/disassemble varying the ionic strength of the medium while recognizing human TfR1 receptor overexpressed in cancer cells. In this paper we present a highly efficient, large scale purification protocol mainly based on crossflow ultrafiltration, starting from fermented bacterial paste.