The Stem Cells and Organoids facility was created thanks to funding from the University under the 2022 Equipment Call. This infrastructure, housed in the basement of the General Physiology building (CU026), consists of an integrated platform for the generation, cultivation, differentiation, modification using genome editing techniques and characterization of stem cells and organoids.
The 'cardiosphere' is a 3D cluster of cardiac progenitor cells recapitulating a stem cell niche-like microenvironment with a potential for disease and regeneration modelling of the failing human myocardium. In this multicellular 3D context, it is extremely important to decrypt the spatial distribution of cell markers for dissecting the evolution of cellular phenotypes by direct quantification of fluorescent signals in confocal microscopy.
© Università degli Studi di Roma "La Sapienza" - Piazzale Aldo Moro 5, 00185 Roma
