Native MS is a non-denaturing technique which allows to preserve noncovalent interactions and quaternary protein structure in the gas phase. Unlike conventional denaturing MS, which often includes organic solvents and low pH, native MS uses volatile, aqueous buffers at near physiological pH and gentler temperatures and voltages. This enables the study of protein-protein interactions, protein-ligand interactions, subunit architecture, and complex stoichiometry. Native MS is compatible with a wide mass range (from small molecules to large multiprotein complexes) and requires only few microliters of sample at high nM to low uM concentration. This is a label free technique and prevents potential issues related to
labelling or immobilization which may alter the intrinsic properties of proteins or their interactions. The Q Exactive UHMR represents the latest advance in native MS technology allowing efficient transmission of large and heterogeneous protein complexes and resolution of small mass differences. The resolution is substantially better than other
instruments, and its sensitivity is orders of magnitude higher. Compared to ToF-based instruments, the Q Exactive has incomparable transmission and sensitivity and, thanks to the latest advancements in the Orbitrap technology, it bridged mass range gap. Given its extended mass range, it allows the transmission and analysis of complexes up to MegaDalton size.
Italian Centre for Native Mass Spectrometry - INCENTIVE MS
Responsabile dell'Attrezzatura
Contatti
cognome | nome | |
---|---|---|
Mai | Antonello |
Struttura
DIPARTIMENTO DI CHIMICA E TECNOLOGIE DEL FARMACO
Descrizione
ERC scientific sector
LS1_1
Anno di collaudo
2022
Stato dell'attrezzatura
In funzione
Nome e acronimo del laboratorio o locale che ospita l'attrezzatura
Lab. 8
Edificio
CU020 - Nuovi Laboratori di Chimica Farmaceutica Farmacia e Medicina
Pagina web laboratorio/attrezzatura
Parole chiave
high resolution mass spectrometry