Platform for the Hypoxic Analysis of Cell Behavior (HYP-ACB) including Seahorse instrument

Responsabile dell'Attrezzatura

The Hyp-ACB facility is a complex infrastructure able to measure, under normoxic and hypoxic conditions, the metabolic activity of living cells, with particular attention to the respiratory activity of mitochondria, in parallel with analysis of gene expression. The facility allows monitoring cell metabolism and gene expression under variable oxygen tensions, thus reproducing more closely the cell or tissue microenvironment(s) in both physiological and pathological conditions.

Understanding how metabolism and gene expression can be turned on or off in a concerted manner allows unravelling the molecular mechanisms underlying physiological metabolic homeostasis and what goes awry in disease. It will also provide information on how biological systems react to environmental stimuli. Possible applications of this technology include multiple areas of biological research, spanning from molecular oncology, cardiovascular diseases, immunology, neurodegeneration, metabolic disorders, ageing, stem cells,tissue engineering, host-pathogen interactions, toxicology, drug discovery, environmental biology, bioengineering, nutrition etc.

The Hyp-ACB laboratory includes the following key equipment :

  • Seahorse XFe96 Agilent

  • Hypoxic cabin Don Whitley  mod. i2 Workstation

  • Sterile hood Thermo Scientific MSC ADVANTAGE 1.2

  • Incubator Thermo Scientific FORMA Steri-cycle 250i

  • Microscope EVOS XL CORE Invitrogen

  • Real-Time PCR Applied Biosystems QuantStudio 3

ERC scientific sector
Anno di collaudo
Stato dell'attrezzatura
In funzione
Nome e acronimo del laboratorio o locale che ospita l'attrezzatura
Room S32/S33
CU027 - Fisiologia Umana Farmacia e Medicina
Parole chiave
Servizi offerti
The Hyp-ACB will perform two major analyses under controlled oxygen tension: • Seahorse XFe96 based analyses of mitochondrial function, glycolysis and fuels. • Gene expression profiling of Core Gene Set, related to energy and redox metabolism. Custom Gene expression profiling will be available upon request. A simple fee-for-service model is difficult to apply given the complexity of the platform, and Hyp- ACB is structured for extensive advising and collaboration. Training includes experimental design and execution and analysis of data. Plasticware, media and reagents, and cell lines will be provided by the end-users, unless otherwise agreed. In general, 3 different types of access are planned: BASIC - Access by expert users (only instruments usage) INTERMEDIATE - Access by non expert users (materials, instruments and sample handling by Facility Staff) ADVANCED - Access by non expert users (as for INTERMEDIATE plus experimental design and data analysis by facility Staff)

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