NMR-based metabolomics Laboratory of Sapienza (NMLab)

Responsabile dell'Attrezzatura

Contatti

cognome	nome	e-mail
Miccheli	Alfredo	alfredo.miccheli@uniroma1.it
Capuani	Giorgio	giorgio.capuani@uniroma1.it

Struttura

DIPARTIMENTO DI CHIMICA

Descrizione

The JEOL JNM-ECZ 600R spectrometer is equipped with a 14.09 Tesla magnet, corresponding to the resonance frequency of ¹H of 600 MHz. For the specific needs of the metabolomic analysis, a 30-position autosampler which can be cooled down to -20°C and a cryoprobe was included in the supply contract.

Instrumental characteristics

SPECTROMETER

Three channels console.
Frequency range: 5 to 645 MHz.
Fully automatic autotune and matching control of all nuclei in the probe range.
Field gradient amplifier, Z axis: 30 A.
Variable temperature control, temperature range: from -170 to 250°C.
Matrix shim system, shim controller, shim driver and shim coil.
Amplifier (RF power monitor, preamplifier).
Low noise and high sensitivity of preamplifiers for high and low frequencies. Automatic relay switching for ²H gradient shimming.
Automatic and manual shimming.

SUPERCONDUCTOR MAGNET 600 MHz

14.09 Tesla magnetic field.
Cryogenic liquid level meter (helium and nitrogen).
Low cryogenic consumption: He refill ≥ 200 days, N₂ refill ≥ 15 days.
Magnet shielding from the center of the magnet: 5 Gauss radial: ≤0.7 m, 5 Gauss axial: ≤1.4 m.
High efficiency anti-vibration system specific for the magnet.
High efficiency external noise suppression system (≥99%).

600 MHz 5 mm FG/RO DIGITAL AUTOTUNE PROBE

Observable and irradiable nuclei: ¹H, ¹⁹F, from ³¹P to ¹⁵N.
Lock system on the ²H signal, easily obtainable without interacting with the instrument hardware (no rewiring).
Guaranteed mono and two-dimensional heteronuclear experiments: HMQC, HSQC, HMBC, DOSY, NOESY, HOESY, COSY, ROESY also in the gradient versions.
Heteronuclei selection by software.
Temperature range: from -100 to + 150°C.

DATA MANAGEMENT SYSTEM

ECZ 5 program for the spectrometer management.
Delta software for the acquisition and processing of data in 1, 2 and 3 dimensions.
Unlimited number of licenses for off-line analysis and graphs.

ERC scientific sector

LS1_10

Anno di collaudo

2020

Stato dell'attrezzatura

In funzione

Nome e acronimo del laboratorio o locale che ospita l'attrezzatura

NMR-based metabolomics of Sapienza (NMLab)

Edificio

CU014 - Chimica - Cannizzaro Scienze Matematiche Fisiche e Naturali

Pagina web laboratorio/attrezzatura

Link al sito del laboratorio

Parole chiave

1H NMR-based metabolomics

Link al regolamento di utilizzo:

Link al regolamento di utilizzo

Servizi offerti

The main activities of the Laboratory are: Characterization of the metabolic profiles (phenotypes) of plant and animal living organisms Characterization of the metabolic profiles of microbial, plant, animal and human cells in relation to genetic mutations or biotic or abiotic perturbations; Identification of new biomarkers to improve clinical diagnosis and prognosis; Identification of new metabolic targets in different human pathologies; Evaluation of the effect of pharmacological, dietary or behavioral interventions on human and animal health; Evaluation of possible side effects of drug treatments; Evaluation of the interactions among diet, intestinal microbiota and human and animal metabolism in physiological and pathological states; Customization of pharmacological and/or nutritional interventions on the basis of the metabolic phenotype of the individual (Precision Medicine and Nutrition); Carrying out the integrated characterization of plant foods in relation to origin, soil and climatic and agronomic conditions; Identification of bioactive compounds in food; Designing functional foods and evaluation of their impact on health. Evaluation of the steps of transformation of food products for process optimization Evaluation of the steps of processes involved in the circular agricultural economy The laboratory provides the necessary structures and skills for the preparation of samples for metabolomics analysis, acquisition of spectra, their processing and subsequent data analysis. The characteristics of the JNM-ECZ 600R spectrometer do not limit its application to metabolomic analysis only, but rather allow its use in numerous other research topics such as, for example, the determination of molecular structure, the composition of mixtures and every application where high resolution mono and two-dimensional NMR spectra are needed.