3D cell culture

Organoid Lab

Organoid Lab

The research group is focused on establishing advanced culture systems, including organoids and primary cell cultures, to better understand host–microorganism interactions. By recreating key features of human tissues in vitro, these models allow the team to explore infection dynamics, immune responses, and the mechanisms that drive disease progression in a controlled and physiologically relevant environment. The research groupls works closely with clinical partners to develop preclinical models that can support translational research and contribute to improved patient care.

Facility Stem Cells and Organoids (SCO)

Italiano

La facility Stem Cells and Organoids è nata grazie ad un finanziamento di Ateneo nell’ambito del Bando Attrezzature 2022. 
Questa infrastruttura, ospitata al piano seminterrato della sede di Fisiologia Generale (CU026), consiste in una piattaforma integrata per la generazione, la coltivazione, il differenziamento, la modifica mediante tecniche di genome editing e la caratterizzazione di cellule staminali e organoidi.

Automated segmentation of fluorescence microscopy images for 3D cell detection in human-derived cardiospheres

The 'cardiosphere' is a 3D cluster of cardiac progenitor cells recapitulating a stem cell niche-like microenvironment with a potential for disease and regeneration modelling of the failing human myocardium. In this multicellular 3D context, it is extremely important to decrypt the spatial distribution of cell markers for dissecting the evolution of cellular phenotypes by direct quantification of fluorescent signals in confocal microscopy.

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