Ricerc@Sapienza

Pectin is synthesized in a highly methylesterified form in the Golgi cisternae and partially de-methylesterified in muro by pectin methylesterases (PMEs). Arabidopsis thaliana produces a local and strong induction of PME activity during the infection of the necrotrophic fungus Botrytis cinerea. AtPME17 is a putative A. thaliana PME highly induced in response to B. cinerea. Here, a fine tuning of AtPME17 expression by different defence hormones was identified.

Adventitious roots (ARs) are post-embryonic roots formed in planta by tissues of the primary root in secondary vascular structure and by tissues of the aerial organs. Indole-3-acetic acid (IAA), and its natural precursor indole-3-butyric acid (IBA), control AR formation in planta and in vitro, however IBA roles have to be elucidated. Arabidopsis thin cell layers (TCL) consist of stem inflorescence tissue external to the vascular system and 10 microM IBA applied with 0.1microM Kinetin induce AR formation from stem TCL.

Adventitious roots (ARs) are post-embryonic roots essential for plant survival and vegetative propagation via cuttings. In different types of explants, the auxin indole-3-acetic acid (IAA), and its natural precursor indole-3-butyric acid (IBA), when applied exogenously, are the main inducers of AR formation. In many cases, exogenous IBA is more effective in inducing ARs than exogenous IAA, but the reason needs investigation. Arabidopsis thaliana thin cell layers (TCLs) consist of stem inflorescence tissues external to the vascular system.

A screening strategy using micropropagation glass tubes with a gradient of distances between germinating seeds
and a metal-contaminated medium was used for studying alterations in root architecture and morphology of
Arabidopsis thaliana treated with cadmium (Cd) and zinc (Zn) at the concentration of 10–20 ?M and 100–200 ?M,
respectively. Metal concentrations in plant shoots and roots were measured by quadrupole inductively coupled
plasma mass spectrometry. After 21 days from germination, all plants in the tubes were scanned at high resolution

Alzheimer's disease (AD) is the most common neurodegenerative disorder and the primary form of dementia in the elderly. One of the main features of AD is the increase in amyloid-beta (Aβ) peptide production and aggregation, leading to oxidative stress, neuroinflammation and neurodegeneration. Polyphenols are well known for their antioxidant, anti-inflammatory and neuroprotective effects and have been proposed as possible therapeutic agents against AD.

In Arabidopsis, stamen elongation, which ensures male fertility, is controlled by the auxin response factor ARF8, which regulates the expression of the auxin repressor IAA19. Here, we uncover a role for light in controlling stamen elongation. By an extensive genetic and molecular analysis we show that the repressor of light signaling COP1, through its targets HY5 and HYH, controls stamen elongation, and that HY5 - oppositely to ARF8 - directly represses the expression of IAA19 in stamens.

Small molecule modulators of the Endoplasmic Reticulum glycoprotein folding quality control (ERQC) machinery have broad-spectrum antiviral activity against a number of enveloped viruses and have the potential to rescue secretion of misfolded but active glycoproteins in rare diseases. In vivo assays of candidate inhibitors in mammals are expensive and cannot be afforded at the preliminary stages of drug development programs.

Plants offer a simpler and cheaper alternative to mammalian animal models for the study of endoplasmic reticulum glycoprotein folding quality control (ERQC). In particular, the Arabidopsis thaliana (At) innate immune response to bacterial peptides provides an easy means of assaying ERQC function in vivo.

A key question in biology is to understand how interspecies morphological diversities originate. Plant roots present a huge interspecific phenotypical variability, mostly because roots largely contribute to adaptation to different kinds of soils. One example is the interspecific cortex layer number variability, spanning from one to several.

How do genes modify cellular growth to create morphological diversity? We study this problem in two related plants with differently shaped leaves: Arabidopsis thaliana (simple leaf shape) and Cardamine hirsuta (complex shape with leaflets). We use live imaging, modeling, and genetics to deconstruct these organ-level differences into their cell-level constituents: growth amount, direction, and differentiation.