clinical biochemistry

An all-glass microfluidic network with integrated amorphous silicon photosensors for on-chip monitoring of enzymatic biochemical assay

A lab-on-chip system, integrating an all-glass microfluidics and on-chip optical detection, was developed and tested. The microfluidic network is etched in a glass substrate, which is then sealed with a glass cover by direct bonding. Thin film amorphous silicon photosensors have been fabricated on the sealed microfluidic substrate preventing the contamination of the micro-channels. The microfluidic network is then made accessible by opening inlets and outlets just prior to the use, ensuring the sterility of the device.

Metal oxide nanoparticle based electrochemical sensor for total antioxidant capacity (TAC) detection in wine samples

A single-use electrochemical screen-printed electrode is reported based on biomimetic properties of nanoceria particles (CeNPs). The developed tool showed an easy approach compared to the classical spectrophotometric methods reported in literature in terms of ease of use, cost, portability, and unnecessary secondary reagents. The sensor allowed the detection of the total antioxidant capacity (TAC) in wine samples. The sensor has been optimized and characterized electrochemically and then tested with antioxidant compounds occurred in wine samples.

The market of chiral drugs: chiral switches versus de novo enantiomerically pure compounds

This review article is aimed at providing an overview of the current market of chiral drugs by exploring which is the nowadays tendency, for the pharmaceutical industry, either to exploit the chiral switching practice from already marketed racemates or to develop de novo enantiomerically pure compounds. A concise illustration of the main techniques developed to assess the absolute configuration (AC) and enantiomeric purity of chiral drugs has been given, where greater emphasis was placed on the contribution of enantioselective chromatography (HPLC, SFC and UHPC).

Nicotine increases colon cancer cell migration and invasion through epithelial to mesenchymal transition (EMT): COX-2 involvement

Cigarette smoking is a recognized risk factor for colon cancer and nicotine, the
principal active component of tobacco, plays a pivotal role in increasing colon cancer
cell growth and survival. The aim of this study was to determine the effect of
nicotine on cellular Caco-2 and HCT-8 migration and invasion, focusing on epithelial
to mesenchymal transition (EMT) induction, and COX-2 pathway involvement. In
both these cell lines, treatment with nicotine increased COX-2 expression and the

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