enantioselective high performance liquid chromatography (eHPLC)

Fast- and Ultra-Fast liquid chromatography and online circular dichroism and fluorimetric detections, U/HPLC CD-PDA-FP

Lingua Italiano

Ultra-high performance liquid chromatograph (UHPLC) equipped with a manual injector and coupled with multi detectors: photodiode array PDA, fluorimetric detector FLD and electronic circular dichroism ECD detector. This equipment allows fast and ultra-fast separations with high sensitivity and high resolution. In addition looking to the only ECD, the acquisition of UV/CD spectrum for each molecules can be obtained.

Investigation of free seleno-amino acids in extra-virgin olive oil by mixed mode solid phase extraction cleanup and enantioselective hydrophilic interaction liquid chromatography-tandem mass spectrometry

An analytical method for determining seleno-methionine (SeMet), methyl-seleno-cysteine and seleno-cystine in extra-virgin olive oil (EVOO) was developed and validated. EVOO sample (15 g) was diluted with hexane, extracted with methanol/water 80:20 (v/v), and cleaned up by a reversed phase/strong cation exchange solid phase extraction. Analysis was performed by chiral hydrophilic interaction liquid chromatography-tandem mass spectrometry.