Ricerc@Sapienza

A relationship between dysbiotic gut microbiome and chronic kidney disease (CKD) has
been recently documented; it contributes to CKD-related complications, including cardiovascular
disease. Aim: We tested how a low-protein diet (LPD)—with or without oral inulin supplementation
as a prebiotic—modulates some inflammatory, atherosclerosis and endothelial dysfunction indices
and nutritional markers, as well as psychocognitive functions in CKD patients. We conducted a

Coffee wastes and by-products from coffee industry are a source of contamination and a serious environmental problem, being mainly unutilized, discharged to the environment or burned. This promoted the interest of researchers in the characterization of wastes and by-products as coffee silverskin (CS, the tegument covering the coffee beans) to evaluate the possibility of reusing them.

Bacterial canker of Actinidia, caused by the bacterium Pseudomonas syringae pv. actinidiae (Psa), is the most serious disease of these plants worldwide. Leaves of three species of Actinidia, namely A. chinensis var. chinensis, A. chinensis var. deliciosa and A. arguta, having different degrees of tolerance to Psa, were analyzed by Nuclear Magnetic Resonance spectroscopy. Aqueous extracts of leaves were studied and several metabolites, classified as organic acids, amino acids, carbohydrates, phenols and other metabolites, were identified by 1D and 2D NMR experiments and quantified.

The chemical composition of the inflorescences from four Cannabis sativa L. monoecious cultivars (Ferimon, Uso-31, Felina 32 and Fedora 17), recently introduced in the Lazio Region, was monitored over the season from June to September giving indications on their sensorial, pharmaceutical/nutraceutical proprieties. Both untargeted (NMR) and targeted (GC/MS, UHPLC, HPLC-PDA/FD and spectrophotometry) analyses were carried out to identify and quantify compounds of different classes (sugars, organic acids, amino acids, cannabinoids, terpenoids, phenols, tannins, flavonoids and biogenic amines).

Bamano, King Creole, Sugarland, and DulceMiel hybrid tomato cultivars have been recently introduced in the Lazio area (Central Italy) to expand and valorize the regional/national market. Tomatoes from these cultivars, together with tomatoes from the native Fiaschetta cultivar, were sampled at the proper ripening time for the fresh market and characterized to obtain and compare their metabolite profiles. The Bligh-Dyer extraction protocol was carried out, and the resulting organic and hydroalcoholic fractions were analyzed by high-field Nuclear Magnetic Resonance (NMR) spectroscopy.