metabolomic analysis

Laboratorio Chimica degli Alimenti - FOODCHEMLAB

Laboratorio Chimica degli Alimenti - FOODCHEMLAB

The research activity of FoodChemLab is mainly focused on the study of food and natural matrices carried out using Nuclear Magnetic Resonance (NMR) methodologies. Together with her research group, prof. Luisa Mannina has developed an analytical protocol for the determination of the metabolic profile of the investigated matrices by means of high-field NMR spectroscopy and, according to the specific problem, chemometric analysis.

Phytochemical analysis and effects on ingestive behaviour of a caralluma fimbriata extract

Caralluma fimbriata Wall. is currently used as a "natural slimming" food supplement, likely due to its content in pregnane glycosides. In the present study, a commercially available Caralluma fimbriata extract (Slimaluma®; CFE, 100 mg/kg) has been evaluated for its ability to affect the ingestive behaviour in female rats, also with reference to the modulation of the brain neuropeptides NPY and ORX.The interference of CFE with ?-amylase and lipase enzymes has been investigated in vitro, as possible peripheral mechanism of action.

The dialogue between died and viable cells: in vitro and in vivo bystander effects and 1H-NMR-based metabolic profiling of soluble factors

The bystander effect (BE) is an important biological phenomenon that induces damages in distant and not directly affected by a chemical/physical stress cells. This effect, well known in ionizing radiation treatment, relies on reactive signals released by exposed cells and transmitted via cell–cell interaction or culture medium. In this study, cycloheximide (CHX)-induced apoptotic U937 cells and untreated THP-1 cells were chosen to investigate the chemical-induced BE.

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