Ricerc@Sapienza

In this paper, we present the first highly porous gold (h-PG) microneedles-based second-generation biosensor for minimally invasive monitoring of glucose in artificial interstitial fluid (ISF). A highly porous microneedles-based electrode was prepared by a simple electrochemical self-templating method that involves two steps, gold electrodeposition and hydrogen bubbling at the electrode, which were realized by applying a potential of −2 V versus a saturated calomel electrode (SCE).

Many research efforts over the last decade have been devoted to the development of microneedle-based diagnostic devices for minimally invasive transdermal biosensing and for long-term health monitoring. Transdermal biosensing via microneedle allows the development of minimally invasive easy-to-use point-of-care biodevices. The main objective of this short review is to provide a general overview of the most immediate and relevant progress in microneedle-based transdermal biosensing in the last five years. A critical analysis of the recent literature is finally presented.

Here we report the first mediated microneedles-based biosensor for minimally invasive continuous sensing of lactate in the dermal interstitial fluid (ISF). To further demonstrate the capability of microneedle arrays as second generation biosensors we have functionalized gold microneedles with nanocarbons at which mediated electron transfer of lactate oxidase takes place.

Here we report the first mediated pain free microneedle‐based biosensor array for the continuous and simultaneous monitoring of lactate and glucose in artificial interstitial fluid (ISF). The gold surface of the microneedles has been modified by electrodeposition of Au‐multiwalled carbon nanotubes (MWCNTs) and successively by electropolymerization of the redox mediator, methylene blue (MB).