Ricerc@Sapienza

Here we report the first mediated microneedles-based biosensor for minimally invasive continuous sensing of lactate in the dermal interstitial fluid (ISF). To further demonstrate the capability of microneedle arrays as second generation biosensors we have functionalized gold microneedles with nanocarbons at which mediated electron transfer of lactate oxidase takes place. In particular the gold surface of the microneedles electrode has been modified in 3 subsequent steps: i) electrodeposition of Au-multiwalled carbon nanotubes (MWCNTs); ii) electropolymerization of the mediator, methylene blue (MB); iii) immobilization of the enzyme lactate oxidase (LOX) by drop-casting procedure. The resulting microneedle-based LOX biosensor displays an interference-free lactate detection without compromising its sensitivity, stability, selectivity and response time. The performance of the microneedle array, second generation biosensor for lactate detection was assessed in artificial interstitial fluid and in human serum, both spiked with lactate. The results reveal that the new microneedles lactate sensor holds interesting promise for the development of a real-time monitoring device to be used in sport medicine and clinical care.