Ricerc@Sapienza

A combined label-free and fluorescence surface optical technique was used to quantify the mass deposited in binary biomolecular coatings. These coatings were constituted by fibronectin (FN), to stimulate endothelialization, and phosphorylcholine (PRC), for its hemocompatibility, which are two properties of relevance for cardiovascular applications. One-dimensional photonic crystals sustaining a Bloch surface wave were used to characterize different FN/PRC coatings deposited by a combination of adsorption and grafting processes.

We report on the development of a biosensing platform that combines label-free and fluorescence based detection on
disposable Bloch surface wave biochips. This system is applied to the detection of the HER2-neu/ErbB2 clinical
biomarker related to breast cancer development. We first describe the design and fabrication of the BSW biochips as
well as the principle of operation of the optical reading instrument. Then, the approaches for surface functionalization