Label-free and fluorescence photonic crystal biochips for early cancer biomarker detection
04 Pubblicazione in atti di convegno
Sinibaldi Alberto, Danz Norbert, Sepe Elisabetta, Munzert Peter, Occhicone Agostino, Allegretti Matteo, Giordani Elena, Giacomini Patrizio, Michelotti Francesco
DOI: 10.1117/12.2514310
We report on the development of a biosensing platform that combines label-free and fluorescence based detection on
disposable Bloch surface wave biochips. This system is applied to the detection of the HER2-neu/ErbB2 clinical
biomarker related to breast cancer development. We first describe the design and fabrication of the BSW biochips as
well as the principle of operation of the optical reading instrument. Then, the approaches for surface functionalization
and immobilization of proteins for specific detection on the biochips are discussed. Finally, experimental results on a
sandwich immunoassay for ErbB2 detection in cell lysates are presented.