Real time qPCR
The Bio-Rad CFX Connect Real-Time System allows for dual target analysis and has excellent thermal cycling characteristics.
The Bio-Rad CFX Connect Real-Time System allows for dual target analysis and has excellent thermal cycling characteristics.
This paper presents a lab-on-chip system suitable for real-time polymerase chain reaction (RTPCR) to quantify the number of DNA copies in samples, taking advantage of the combination of thin film technologies on the same glass substrate. A thin film metal heater, deposited on one side of a glass substrate, provides the thermal energy for the DNA treatment, while amorphous silicon (a-Si:H) photosensors, deposited on the other glass side, monitor the DNA amplification. Indeed, the glass is optically coupled with another glass hosting the microfluidic network, where the PCR occurs.
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