Lab-on-Chip system based on thin film technologies for real-time PCR
04 Pubblicazione in atti di convegno
Caputo Domenico, Petrucci Giulia, Costantini Francesca, Lovecchio Nicola, Nardecchia Marco, Nascetti Augusto, DE CESARE Giampiero
This paper presents a lab-on-chip system suitable for real-time polymerase chain reaction (RTPCR) to quantify the number of DNA copies in samples, taking advantage of the combination of thin film technologies on the same glass substrate. A thin film metal heater, deposited on one side of a glass substrate, provides the thermal energy for the DNA treatment, while amorphous silicon (a-Si:H) photosensors, deposited on the other glass side, monitor the DNA amplification. Indeed, the glass is optically coupled with another glass hosting the microfluidic network, where the PCR occurs.