Ricerc@Sapienza

By using the Inverted Chirality Columns Approach (ICCA) we have developed an enantioselective UHPSFC method to determine the enantiomeric excess (ee) of (-)-?(9)-THC in medicinal marijuana (Bedrocan®). The ee was high (99.73%), but the concentration of the (+)-enantiomer (0.135%) was not negligible, and it is worth a systematic evaluation of bioactivity.

This review presents advancements in sample preparation and liquid chromatographic methods made during the last 10 years for the fat-soluble vitamin (FSV) analysis in foods. Since activity. and bioavailability of organic micronutrients (MOs) depend on the form in which they are present in a food, special emphasis has been given to the most recent techniques, instruments and approaches which have allowed the extraction and separation of various vitamin homologues as well as the comprehensive MO profiling in foods.

The application of a novel chromatographic approach to therapeutic peptides bearing basic amino acids in their structure allowed unprecedented resolution of their related impurities (including epimeric isobaric ones), resulting in a superior analytical tool for the evaluation of the quality of these drugs in the market.