The development of selective, ecofriendly, low cost, stable and recycling biosensors for molecules and biomolecules detection in different environments such as atmosphere, food industry or in clinical analyses is strongly required. Among the most studied bio-components, enzymes play a leading role, being a class of biological recognition elements which are selective and sensitive. In this regard, the fabrication of enzyme based biosensors has received a huge interest in the last years. In particular many laccase based biosensors have been developed since this oxidoreductase enzyme is used in different fields due to its robustness and cheapness. In the fabrication process, enzyme immobilization strategies appear as a key factor to develop an efficient tool with appropriate performances. We are currently manufacturing new promising amperometric laccase-based biosensors with unprecedented reuse and storage capabilities by using the ambient electrospray deposition (ESD) methodology as immobilization technique. These new devices will be characterized electrochemically and the effects of metal nanoparticles deposition on the surfaces of commercial screen printed electrodes will be also investigated. Thanks to an interdisciplinary team, the main goal of this project is to extend the knowledge of these high resistant biosensors at the molecular level by using different approaches, microscopies and analytical tools to prove how the ESD immobilization technique is suited to manufacture high performance devices in term of storage and reuse.
