Histone demethylases (HDMs) have a prominent role in epigenetic regulation and are emerging as potential
therapeutic cancer targets. In particular, HDMs of class 5 (KDM5) ,also known as JARID, are over-expressed in different types of cancers and have a prominent role in cancer cells ploriferation and DNA damage repair. In particular, KDM5b (JARID 1B) is up-regulated in 90% of human breast cancers. In order to understand its role in breast cancer cells physiology, we propose:
a - to use different chemical inhibitors of KDM5 catalytic activity and to test their effects on transcriptome, cell survival and DNA damage repair.
b - to study the post-transcriptional regulation of JARID 1B expression by miRNAs potentially targeting the protein and significantly modulated in breast cancer cells and their effects on its functional activities
