Proteomic approach to identify periplasmic protein(s) associated with carbapenem resistance in the Acinetobacter baumannii model strain AB5075

Proponente Carlo Zagaglia - Ricercatore
Sottosettore ERC del proponente del progetto
Componenti gruppo di ricerca
Componente Qualifica Struttura Categoria
Daniela Scribano altro Dip.di Scienze Mediche, Orali e Biotecnologiche, Università G. D'Annunzio, Chieti Altro personale Sapienza o esterni
Laura Di Francesco assegnista Unità di Microbioma Umano, Ospedale Pediatrico Bambino Gesù, IRCCS, Roma Altro personale Sapienza o esterni

Antibiotic resistance has become a global alarm comparable to that of climate changes. The World Health Organization listed Acinetobacter baumannii as one of the microorganism of critical concern for its resistance to carbapenems. The oxacillinase OXA-23 represents the dominant ß-lactamase enzyme to confer resistance to carbapenems among A. baumannii clinical isolates worldwide. It was shown recently that OXA-23 interacts with several protein partners located within the outer membrane and the periplasm. Based on the hypothesis that periplasm interactors might be required to support OXA-23 enzymatic activity upon carbapenem exposure, we aimed this project at identifying periplasmic proteins that are upregulated upon addition of carbapenems in the growth medium using a proteomic approach; moreover, we will study the involvement of upregulated proteins in OXA-mediated carbapenem-resistance, fitness and virulence in A. baumannii strain AB5075.


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