Optimization of an in vitro transcription-translation system based on Sulfolobus solfataricus cell lysate
The aim of the project is that to develop an in vitro system which permits the efficient synthesis of proteins in vitro at high temperature. This method is based on the use of an unfractionated cell lysate (S-30) from Sulfolobus solfataricus previously well characterized in our laboratory and the novelty of use it coupled with an in vitro transcription system.
The novelty of this project is based on the use of an essential element corresponding to a strong promoter derived from 16S/23S rRNA-encoding DNA promoter from the archaebacterium Sulfolobus sp. P2 that produces, with high efficiency, specific mRNAs.
The simplicity of the experimental procedure and specific activity of the proteins produced offer a number of possibilities for the study of structure-function relationships of proteins.