Respiratory tract colonization and respiratory microbiota in antibody deficiencies

Anno
2018
Proponente Isabella Quinti - Professore Ordinario
Sottosettore ERC del proponente del progetto
Componenti gruppo di ricerca
Abstract

Disease-specific studies focused on infection risk in primary antibody deficiencies are needed to define strategies for controlling respiratory infections. Little information is available on the rate of airway bacterial carriage and its consequence. In hypogammaglobulinemias, Despite IgG replacement, recurrent respiratory infections are common in PAD possibly leading to chronic lung damage and poor quality of life. Thus, patients are often prescribed antibiotics and/or long-term antimicrobial prophylactic regimens. However, antibiotics influence antimicrobial resistance among airway microbiota. In a recent meta-analysis on patients with chronic lung diseases, 30% of S. pneumoniae showed resistance to macrolides.
In this observational longitudinal study, we will investigate the rate of nasopharyngeal and oropharyngeal mucosal carriage detected by cultural methods and Real-time-PCR (RT-PCR), the antimicrobial susceptibility patterns and the antibiotic usage in 300 adult patients with Common Variable Immunodeficiency (http://esid.org/Working-Parties/Registry/Diagnosis-criteria). Additionally, we will verify whether colonization would be associated with risk for respiratory infections. Rate of respiratory infections, clinical data and days of antibiotic usage in the six months prior to swabs collection will be recorded. Sample collection and identification of bacteria will be performed according to the WHO standard procedures by cultural and molecular methods on swabs collected on the same day. For both bacterial species, minimum inhibitory concentrations of antimicrobial agents will be determined by the interpretative criteria based on EUCAST breakpoints (http://www.eucast.org/clinical breakpoints/). Diversity and type of airways colonizing microbiota will be performed by amplification of the V3-V4 16S rRNA gene region. After swabs collection, respiratory infections will be recorded for additional 6 months.

ERC
LS6_2, LS6_6, LS6_7
Keywords:
IMMUNOLOGIA CLINICA, MICROBIOLOGIA, ANTIBIOTICO RESISTENZA, IMMUNITA¿ ADATTATIVA, MALATTIE INFETTIVE

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