Application of DPPH assay for assessment of particulate matter reducing properties
Different acellular assays were developed to measure particulate matter’s (PM) oxidative potential (OP) to predict the PM ability to generate oxidative stress in living cells and organisms. However, there are still fundamental unresolved issues regarding the influence of assay’s design and the tests’ operative condition on OP obtained results. An additional important point is related to the stability of the species: short-life oxidant species can react and redox equilibria among PM native species could occur during both the sample storage and the extraction phase.