Primary cultures

A Challenging Task—How to Successfully Separate Theca and Granulosa Cells: A Mandatory Step for Investigating Ovary Steroidogenesis

The reciprocal interaction in between theca (TCs) and granulosa (GCs) cells plays a pivotal role in al-lowing ovary to display a wide range of physiological functions, including steroidogenesis and reproduction capabilities. For instance, changes in the respective proportion among GCs and TCS have been correlated to progression or recovery from Polycystic Ovary Syn-drome (Bevilacqua, et al. 2019). To investigate how these cells cooperate, it is important to understand how they work in isolation.

ATP-evoked intracellular Ca2+ transients shape the ionic permeability of human microglia from epileptic temporal cortex

BACKGROUND: Intracellular Ca2+ modulates several microglial activities, such as proliferation, migration, phagocytosis, and inflammatory mediator secretion. Extracellular ATP, the levels of which significantly change during epileptic seizures, activates specific receptors leading to an increase of intracellular free Ca2+ concentration ([Ca2+]i).

Role of c-MET inhibitors in overcoming drug resistance in spheroid models of primary human pancreatic cancer and stellate cells

Pancreatic stellate cells (PSCs) are a key component of tumor microenvironment in pancreatic ductal adenocarcinoma (PDAC) and contribute to drug resistance. c-MET receptor tyrosine kinase activation plays an important role in tumorigenesis in different cancers including PDAC. In this study, effects of PSC conditioned medium (PCM) on c-MET phosphorylation (by immunocytochemistry enzyme-linked immunosorbent assay (ELISA)) and drug response (by sulforhodamine B assay) were investigated in five primary PDAC cells.

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