Application of Simvastatin on Regenerative Endodontic procedures

Anno
2019
Proponente Maurizio Bossu' - Professore Associato
Sottosettore ERC del proponente del progetto
LS7_10
Componenti gruppo di ricerca
Componente Categoria
Gaetano Ierardo Componenti strutturati del gruppo di ricerca / Structured participants in the research project
Gianni Di Giorgio Dottorando/Assegnista/Specializzando componente non strutturato del gruppo di ricerca / PhD/Assegnista/Specializzando member non structured of the research group
Carlo Di Paolo Componenti strutturati del gruppo di ricerca / Structured participants in the research project
Gianluca Gambarini Componenti strutturati del gruppo di ricerca / Structured participants in the research project
Luca Testarelli Componenti strutturati del gruppo di ricerca / Structured participants in the research project
Componente Qualifica Struttura Categoria
Carlo Prati Professore Ordinario Università degli Studi di Bologna Altro personale aggregato Sapienza o esterni, titolari di borse di studio di ricerca / Other aggregate personnel Sapienza or other institution, holders of research scholarships
Sandro Rengo Professore Ordinario Università di Napoli Federico II Altro personale aggregato Sapienza o esterni, titolari di borse di studio di ricerca / Other aggregate personnel Sapienza or other institution, holders of research scholarships
Mario Trottini Professore titolare Universidad de Alicante (Spagna) Altro personale aggregato Sapienza o esterni, titolari di borse di studio di ricerca / Other aggregate personnel Sapienza or other institution, holders of research scholarships
Giuseppe Scapigliati Professore Ordinario Università degli Studi della Tuscia (VT) Altro personale aggregato Sapienza o esterni, titolari di borse di studio di ricerca / Other aggregate personnel Sapienza or other institution, holders of research scholarships
Abstract

The regenerative endodontic procedures (REPs) guidelines offer a valid clinical procedure proposing the use of a combination of antimicrobials and irrigants, no canal walls instrumentation, induced apical bleeding to form a blood clot and a tight seal into the root canal to promote healing. This procedure still presents a certain unpredictability. Drawbacks such as lack of real tissue regeneration and maintained signs or symptoms are to be described.

Three factors are needed to achieve upregulation of cellular synthesis and the formation of new tissue: A permissive environment, cells capable of secreting extracellular matrices and the release of molecular signals for cell interaction. Recent studies show that, statins, a family of lipid-lowering agents, have anti-inflammatory properties and suppress the progression of apical periodontitis. Simvastatin induces odontoblastic differentiation, inhibit osteoclast differentiation in periapical lesions and promotes calcified matrix formation.

Dental pulp stem cells (DPSC) may rebuild the lost pulp tissue in the canal and give rise to replacement odontoblasts for substituting the damaged primary ones, being totipotential stem cells.

For further investigation we propose to culture this cells exposing them to simvastatin (SIM) and apply them to an in vitro model of artificial pulp chamber (APC) in a group of a DPSC matrix with a SIM supplemented absorbable gelatin sponge, another of cultured blood with a SIM supplemented absorbable gelatin sponge above and a control group.

Testing the SIM infused cells for metabolic activity, ALP activity, calcium deposition, and cell migration helps to understand the action of simvastatin on them. Testing the three APC groups for Live/Dead assay, and different gene expressions by real-time polymerase chain reaction and mineralized matrix deposition is useful to understand how an absorbable gelatin sponge and simvastatin can improve the current REPs guidelines success rate and results.

ERC
LS3_12, LS7_5, LS7_10
Keywords:
ODONTOIATRIA, STOMATOLOGIA, RIGENERAZIONE TISSUTALE, TERAPIA CON CELLULE STAMINALI

© Università degli Studi di Roma "La Sapienza" - Piazzale Aldo Moro 5, 00185 Roma