Ricerc@Sapienza

Background: High-grade serous ovarian cancer (HGSOC) causes 80% of all
ovarian cancer (OC) deaths. In this setting, the role of cancer stem-like cells (CSCs) is still unclear.
In particular, the evolution of CSC biomarkers from primary (pOC) to recurrent (rOC)
HGSOCs is unknown. Aim of this study was to investigate changes in CD133 and aldehyde
dehydrogenase-1 (ALDH1) CSC biomarker expression in pOC and rOC HGSOCs.
Methods: Two-hundred and twenty-four pOC and rOC intrapatient paired tissue samples
derived from 112 HGSOC patients were evaluated for CD133 and ALDH1 expression using
immunohistochemistry (IHC); pOCs and rOCs were compared forCD133 and/orALDH1 levels.
Expression profiles were also correlated with patients’ clinicopathological and survival data.
Results: Some 49.1% of the patient population (55/112) and 37.5% (42/112) pOCs were CD133þ
andALDH1þrespectively.CD133þandALDH1þsamples were detected in 33.9%(38/112) and
36.6% (41/112) rOCs. CD133/ALDH1 coexpression was observed in 23.2% (26/112) and 15.2%
(17/112) of pOCs and rOCs respectively. Pairwise analysis showed a significant shift of CD133
staining from higher (pOCs) to lower expression levels (rOCs) (p < 0.0001). Furthermore, all
CD133 þ pOC patients were International Federation of Gynaecology and Obstetrics
(FIGO)-stage III/IV (p < 0.0001) and had significantly worse progression-free interval (PFI)
(pZ0.04) and overall survival (OS) (pZ0.02). On multivariate analysis, CD133/ALDH1 coexpression
in pOCs was identified as independent prognostic factor for PFI (HR: 1.64; 95%CI: 1.03
e2.60; pZ0.036) and OS (HR: 1.71; 95% CI: 1.01e2.88; pZ0.045). Analysis on 52 pts patients
with known somatic BRCA status revealed that BRCA mutations did not influence CSC
biomarker expression.
Conclusions: The study showed that CD133/ALDH1 expression impacts HGSOC patients’ survival
and first suggests that CSCs might undergo phenotypic change during the disease course
similarly to non stem-like cancer cells, providing also a first evidence that there is no correlation
between CSCs and BRCA status.