Daniela De Biase


Titolo Pubblicato in Anno
Management of chronic stable angina: modern microbiomedical research provides insights into Traditional Chinese Medicine treatments JOURNAL OF CARDIOVASCULAR PHARMACOLOGY 2020
Advanced course trends in enzymology and biocatalysis Advanced Course: Trends in Enzymology and Biocatalysis - Programme and Abstracts 2019
Lethality of Brucella microti in a murine model of infection depends on the wbkE gene involved in O-polysaccharide synthesis VIRULENCE 2019
Biochemical characterization of Glutamate decarboxylase from Mycobacterium tuberculosis Microbial Stress: from Systems to Molecules and Back 2018
Glutaminase activity and glutamine-dependent acid resistance in enteric bacteria 2018
Effect of temperature, pH and plasmids on in vitro biofilm formation in Escherichia coli ACTA NATURAE 2018
The glutaminase-dependent acid resistance system. Qualitative and quantitative assays and analysis of its distribution in enteric bacteria FRONTIERS IN MICROBIOLOGY 2018
On the effect of alkaline pH and cofactor availability in the conformational and oligomeric state of Escherichia coli glutamate decarboxylase PROTEIN ENGINEERING, DESIGN & SELECTION 2017
The yhiM gene codes for an inner membrane protein involved in GABA export in Escherichia coli AIMS MICROBIOLOGY 2017
Glutamate decarboxylase in bacteria The Handbook of Microbial Metabolism of Amino Acids 2017
The Glutaminase-dependent system confers extreme acid resistance to new species and atypical strains of Brucella FRONTIERS IN MICROBIOLOGY 2017
Biochemical characterization of a variant of Escherichia coli glutamate decarboxylase with improved GABA production at alkaline pH. FEMS2017: 7th Congress of European Microbiologists 2017
Role of the glutamine-dependent acid resistance system of Brucella spp. in response to extreme acid stress FEMS 2017, 7th Congress of European Microbiologists 2017
Bicyclic γ-amino acids as inhibitors of γ-aminobutyrate aminotransferase JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY 2016
Convenient syntheses of phosphinic analogues of γ-aminobutyric- and glutamic acids RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY 2016
Acid survival mechanisms in neutralophilic bacteria Stress and environmental regulation of gene expression and adaptation in bacteria 2016
The role of an active site aspartate residue in the catalytic activity of Glutamate decarboxylase from Escherichia coli. The biochemistry and chemistry of biocatalysis: From understanding to design 2016
Glutamate decarboxylase-dependent acid resistance in Brucella spp.. Distribution and contribution to fitness under extremely acidic conditions APPLIED AND ENVIRONMENTAL MICROBIOLOGY 2015
Biochemical and spectroscopic properties of Brucella microti glutamate decarboxylase, a key component of the glutamate-dependent acid resistance system FEBS OPENBIO 2015
The Escherichia coli acid stress response and its significance for pathogenesis ADVANCES IN APPLIED MICROBIOLOGY 2015


  • LS1
  • LS6_7

Interessi di ricerca

  • Microbiology-Molecular Biology: working on the transcriptional regulation and the biochemistry of the glutamate and glutamine-dependent systems, the major acid resistance (AR) systems in Escherichia coli (commensal and pathogenic) and many other enteric bacteria. Several interconnections between different AR systems and other physiological and stress-related processes in E. coli and other bacteria are currently under investigation.
  • Biochemistry: Expert on PLP-dependent enzymes, in particular glutamate decarboxylase (GAD) and GABA-transaminase (GABA-T) of mammalian and bacterial origin. The structural properties and the catalytic mechanism of GAD are investigated via site-directed mutagenesis, expression and purification of recombinant variants, kinetic and spectroscopic studies in combination with structural studies (the latter in collaboration).
  • Biotechnology: Exploiting and engineering GAD for biotech applications, including the synthesis of bioactive molecules. In addition she is also studying the acid stress response in E. coli and other bacterial species for applications in the food and biotech sector (vice-chair of the COST Action CA18113 “Understanding and exploiting the impacts of low pH on micro-organisms”)


antiepileptic drugs
biochemistry, genetics and molecular biology (all)
Escherichia coli protein
glutamate-dependent acid resistanc
PLP-dependent enzym

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