Alessandro Rosa

Pubblicazioni

Titolo Pubblicato in Anno
Single-cell transcriptomics identifies master regulators of neurodegeneration in SOD1 ALS iPSC-derived motor neurons STEM CELL REPORTS 2021
A computational approach to investigate tdp-43 rna-recognition motif 2 c-terminal fragments aggregation in amyotrophic lateral sclerosis BIOMOLECULES 2021
PiggyBac vectors in pluripotent stem cell research and applications Methods in iPSC Technology 2021
Excess TPX2 interferes with microtubule disassembly and nuclei reformation at mitotic exit CELLS 2020
Establishment of an in Vitro Human Blood-Brain Barrier Model Derived from Induced Pluripotent Stem Cells and Comparison to a Porcine Cell-Based System CELLS 2020
Acute conversion of patient-derived Duchenne muscular dystrophy iPSC into myotubes reveals constitutive and inducible over-activation of TGFβ-dependent pro-fibrotic signaling SKELETAL MUSCLE 2020
FUS ALS-causative mutations impair FUS autoregulation and splicing factor networks through intron retention NUCLEIC ACIDS RESEARCH 2020
Trans-generational epigenetic regulation associated with the amelioration of Duchenne Muscular Dystrophy EMBO MOLECULAR MEDICINE 2020
HOTAIRM1 regulates neuronal differentiation by modulating NEUROGENIN 2 and the downstream neurogenic cascade CELL DEATH & DISEASE 2020
Proteomics analysis of FUS mutant human motoneurons reveals altered regulation of cytoskeleton and other ALS-linked proteins via 3′UTR binding SCIENTIFIC REPORTS 2020
Identification of molecular signatures in neural differentiation and neurological diseases using digital color-coded molecular barcoding STEM CELLS INTERNATIONAL 2020
High-throughput screening identifies histone deacetylase inhibitors that modulate GTF2I expression in 7q11.23 microduplication autism spectrum disorder patient-derived cortical neurons MOLECULAR AUTISM 2020
The Aurora-A/TPX2 axis directs spindle orientation in adherent human cells by regulating NuMA and microtubule stability CURRENT BIOLOGY 2020
Conversion of Human Induced Pluripotent Stem Cells (iPSCs) into Functional Spinal and Cranial Motor Neurons Using PiggyBac Vectors JOURNAL OF VISUALIZED EXPERIMENTS 2019
Mutant FUS and ELAVL4 (HuD) aberrant crosstalk in Amyotrophic Lateral Sclerosis CELL REPORTS 2019
Construction of 3D in vitro models by bioprinting human pluripotent stem cells: Challenges and opportunities BRAIN RESEARCH 2019
Inducible SMARCAL1 knockdown in iPSC reveals a link between replication stress and altered expression of master differentiation genes DISEASE MODELS & MECHANISMS 2019
3D bioprinted human cortical neural constructs derived from induced pluripotent stem cells JOURNAL OF CLINICAL MEDICINE 2019
Influence of substrate stiffness on human induced pluripotent stem cells: Preliminary results Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS 2019
Direct conversion of human pluripotent stem cells into cranial motor neurons using a piggyBac vector STEM CELL RESEARCH 2018

ERC

  • LS1_3
  • LS2_4
  • LS3_12
  • LS3_13
  • LS5_11

KET

  • Life-science technologies & biotechnologies

Interessi di ricerca

My field of research concerns the characterization of the molecular mechanisms that underlie cell function during differentiation and development, in order to understand how these mechanisms are altered in pathological conditions and how they can be used in therapy. In particular, my primary interest is the RNA molecule.

We use induced Pluripotent Stem Cells (iPSCs) for the study of neurodegenerative and neurodevelopmental diseases. iPSCs can be derived from patients carrying mutations, modified by gene editing and differentiated in vitro into tissues of interest in conventional monolayer cultures or as 3D organoids. Thus, they provide excellent in vitro model systems for the study of the molecular and cellular basis of diseases of the nervous system.

Keywords

human induced pluripotent stem cells (hiPSC)
microRNA
RNA-binding proteins
motoneuron
amyotrophic lateral sclerosis (ALS)
brain organoid

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