Alessandro Rosa

Pubblicazioni

Titolo Pubblicato in Anno
Culture of Human iPSC-Derived Motoneurons in Compartmentalized Microfluidic Devices and Quantitative Assays for Studying Axonal Phenotypes Methods in Molecular Biology 2022
Novel fragile X syndrome 2D and 3D brain models based on human isogenic FMRP-KO iPSCs CELL DEATH & DISEASE 2021
Small heat-shock protein HSPB3 promotes myogenesis by regulating the lamin B receptor CELL DEATH & DISEASE 2021
FUS-ALS mutants alter FMRP phase separation equilibrium and impair protein translation SCIENCE ADVANCES 2021
ALS-related FUS mutations alter axon growth in motoneurons and affect HuD/ELAVL4 and FMRP activity COMMUNICATIONS BIOLOGY 2021
Editorial: The RNA Revolution in Embryonic Development and Cell Differentiation in Health and Disease FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY 2021
Single-cell transcriptomics identifies master regulators of neurodegeneration in SOD1 ALS iPSC-derived motor neurons STEM CELL REPORTS 2021
A computational approach to investigate tdp-43 rna-recognition motif 2 c-terminal fragments aggregation in amyotrophic lateral sclerosis BIOMOLECULES 2021
PiggyBac vectors in pluripotent stem cell research and applications Methods in iPSC Technology 2021
Excess TPX2 interferes with microtubule disassembly and nuclei reformation at mitotic exit CELLS 2020
Establishment of an in Vitro Human Blood-Brain Barrier Model Derived from Induced Pluripotent Stem Cells and Comparison to a Porcine Cell-Based System CELLS 2020
Acute conversion of patient-derived Duchenne muscular dystrophy iPSC into myotubes reveals constitutive and inducible over-activation of TGFβ-dependent pro-fibrotic signaling SKELETAL MUSCLE 2020
FUS ALS-causative mutations impair FUS autoregulation and splicing factor networks through intron retention NUCLEIC ACIDS RESEARCH 2020
Trans-generational epigenetic regulation associated with the amelioration of Duchenne Muscular Dystrophy EMBO MOLECULAR MEDICINE 2020
HOTAIRM1 regulates neuronal differentiation by modulating NEUROGENIN 2 and the downstream neurogenic cascade CELL DEATH & DISEASE 2020
Proteomics analysis of FUS mutant human motoneurons reveals altered regulation of cytoskeleton and other ALS-linked proteins via 3′UTR binding SCIENTIFIC REPORTS 2020
Identification of molecular signatures in neural differentiation and neurological diseases using digital color-coded molecular barcoding STEM CELLS INTERNATIONAL 2020
High-throughput screening identifies histone deacetylase inhibitors that modulate GTF2I expression in 7q11.23 microduplication autism spectrum disorder patient-derived cortical neurons MOLECULAR AUTISM 2020
The Aurora-A/TPX2 axis directs spindle orientation in adherent human cells by regulating NuMA and microtubule stability CURRENT BIOLOGY 2020
Conversion of Human Induced Pluripotent Stem Cells (iPSCs) into Functional Spinal and Cranial Motor Neurons Using PiggyBac Vectors JOURNAL OF VISUALIZED EXPERIMENTS 2019

ERC

  • LS1_3
  • LS2_4
  • LS3_12
  • LS3_13
  • LS5_11

KET

  • Life-science technologies & biotechnologies

Interessi di ricerca

My field of research concerns the characterization of the molecular mechanisms that underlie cell function during differentiation and development, in order to understand how these mechanisms are altered in pathological conditions and how they can be used in therapy. In particular, my primary interest is the RNA molecule.

We use induced Pluripotent Stem Cells (iPSCs) for the study of neurodegenerative and neurodevelopmental diseases. iPSCs can be derived from patients carrying mutations, modified by gene editing and differentiated in vitro into tissues of interest in conventional monolayer cultures or as 3D organoids. Thus, they provide excellent in vitro model systems for the study of the molecular and cellular basis of diseases of the nervous system.

Keywords

human induced pluripotent stem cells (hiPSC)
microRNA
RNA-binding proteins
motoneuron
amyotrophic lateral sclerosis (ALS)
brain organoid

Laboratori di ricerca

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