The confocal microscope Crest Csrv II Lxis an optical microscope that is based on a technology aimed at significantly increasing the spatial resolution of the sample, eliminating the halos due to the light diffused by the out-of-focus planes of the specimen.
The inverted microscope Nikon Eclipse Ti is equipped with systems for acquisition or live cell imaging [Phase Contrast, fluorescence and Förster resonance energy transfer (FRET)]. It is also equipped with a Micro-incubator for temperature and CO2 environment control.
The "ChemiDoc MP" is a very flexible imaging system, capable of detecting colorimetric, chemiluminescent, fluorescent, red, green, blue and UV signals. It is also compatible with the 48 “Stain Free” technology for the visualization of proteins, immediately after the electrophoretic run.
The isolated heart or Langendorff heart is an in vitro technique used in pharmacology and physiology that allows to examine contractility and heart rate without the problems of in vivo methods.
Nel laboratorio 5 della sezione di Istologia si effettuano: allestimento e mantenimento di colture cellulari, analisi biochimiche (proteine e acidi nucleici) e istologiche (colorazioni su sezioni) su tessuti o cellule derivanti da animali da laboratorio, da biopsie umane o da linee cellulari, nell’ambito della ricerca di base e preclinica sulle seguenti tematiche:
Omeostasi del microambiente immunitario tumorale: meccanismi molecolari e cellulari
Meccanismi molecolari e cellulari del processo metastatico in cellule di melanoma e carcinoma prostatico umane
Nanostring GEOMX Digital Spatial Profiler
Caratteristiche principali
✓ Tecnologia basata su identificazione di regioni di interesse tramite l’impiego di 4 biomarker immunofluorescenti proteici o RNA e successiva conta digitale delle molecole di interesse tramite ibridazione di sonde molecolari multiplex marcate con barcode fluorescenti.
✓ Possibilità di testare fino ad un massimo di 96 marcatori proteici o 1000 RNA contemporaneamente da singola slide FFPE
Variable pressure scanning electron microscopy (VP-SEM, Hitachi SU-3500) is supported by dual energy dispersive X-ray spectroscopy detectors (dEDS) arranged in parallel configuration (Bruker, XFlash® 6|60) able to high sensitivity elemental analysis by their large active area of a 60 mm2 each.VP-SEM-dEDS is able to observe in situ
The equipment is characterized by a Cell Imaging tool combined with a Multi-Mode Reader and an incubator-like system for real-time live cell and longer-term kinetic studies. The instrument should work by multiple advanced combined technologies, including fluorescence and high contrast brightfield imaging, monochromator-based ultraviolet and visible (UV-VIS) absorbance, high performance filter-based fluorescence and luminescence.
Microscopio confocale spettrale ZEISS LSM 800 con 4 Laser su stativo rovesciato ZEISS Axio Observer 7 e sistema in super-risoluzione Airyscan per live-imaging e timelapse.
- Microscopio rovesciato da ricerca Axio Observer per LSM
- completamente motorizzato con passo 10nm (asse Z)
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