Ricerc@Sapienza

Acquisizione spettri UV-vis;  range di assorbanza molto ampio;

Acquisizione spettri di emissione  e di assorbimento di fluorescenza;

Acquisizione spettri di dicroismo circolare UV-visibile;

  The ARP laboratory includes in detail the following equipment:

•  Applied Photophysics single-mixing -SX-18

•  Applied Photophysics Pi-star 180, 

•  Applied Photophysics sequential-mixing DX-17MV

• Tgk Scientific Hi-Tech PTJ-64 capacitor-discharge T-jump apparatus

Despite having an optimal sample for crystallization, obtaining crystals can be an arduous and unpredictable goal. The most important approaches to overcome this bottleneck were the discovery of appropriate precipitants, new methods of preparation of macromolecule samples, new methods of carrying out crystallization experiments but above all the increase of crystallization efficiency through automation.

The instrument is designed for high resolution mass spectrometry of proteins and nucleic acids. 

The source can be operated in positive and negative ion mode using the linear or reflector detector. The saturation free detector technology enables highest dynamic range and superior performance for high-mass detection.

PESCA platform consists in the combination of AFM (with nm resolution) with an electrochemical set-up. This combination  affords simultaneous acquisition of different

types of images (topological activity of electrified surface and correlation with applied electrical potential) unveiling fundamental interfacial properties at nm range

Circular Dichroism spectrometer Jasco J-730 equipped with Peltier thermostatted apparatus

The Hyp-ACB facility is a complex infrastructure able to measure, under normoxic and hypoxic conditions, the metabolic activity of living cells, with particular attention to the respiratory activity of mitochondria, in parallel with analysis of gene expression. The facility allows monitoring cell metabolism and gene expression under variable oxygen tensions, thus reproducing more closely the cell or tissue microenvironment(s) in both physiological and pathological conditions.

Lo spettrometro di massa a trappola ionica lineare LTQ XL fornito di sorgenti ionica ESI e di interfaccia FAIMS è fornito dalla ditta ThermoFisher Scientific. E’ inoltre modificabile per consentire l’ingresso di radiazione laser dal sistema OPO/OPA che si prevede di interfacciare in una fase seguente di integrazione della piattaforma. L'interfaccia FAIMS consentirà di aggiungere una nuova dimensione alle prestazioni della spettrometria di massa.