Giorgio Giardina

Pubblicazioni

Titolo Pubblicato in Anno
The moonlighting RNA-binding activity of cytosolic serine hydroxymethyltransferase contributes to control compartmentalization of serine metabolism NUCLEIC ACIDS RESEARCH 2019
R180T variant of δ-ornithine aminotransferase associated with gyrate atrophy: biochemical, computational, X-ray and NMR studies provide insight into its catalytic features THE FEBS JOURNAL 2019
N-acetylcysteine serves as substrate of 3-mercaptopyruvate sulfurtransferase and stimulates sulfide metabolism in colon cancer cells CELLS 2019
Linking infection and prostate cancer progression: Toll-like receptor3 stimulation rewires glucose metabolism in prostate cells ANTICANCER RESEARCH 2019
Cycloserine enantiomers are reversible inhibitors of human alanine:glyoxylate aminotransferase: implications for primary hyperoxaluria type 1 BIOCHEMICAL JOURNAL 2019
Beyond nitrogen metabolism: nitric oxide, cyclic-di-GMP and bacterial biofilms FEMS MICROBIOLOGY LETTERS 2018
Differential inhibitory effect of a pyrazolopyran compound on human serine hydroxymethyltransferase-amino acid complexes ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS 2018
Insights into the GTP-dependent allosteric control of c-di-GMP hydrolysis from the crystal structure of PA0575 protein from Pseudomonas aeruginosa THE FEBS JOURNAL 2018
The catalytic activity of serine hydroxymethyltransferase is essential for de novo nuclear dTMP synthesis in lung cancer cells THE FEBS JOURNAL 2018
A novel bacterial l-arginine sensor controlling c-di-GMP levels in Pseudomonas aeruginosa PROTEINS 2018
Glucose metabolism in the progression of prostate cancer FRONTIERS IN PHYSIOLOGY 2017
Radiation damage at the active site of human alanine:glyoxylate aminotransferase reveals that the cofactor position is finely tuned during catalysis SCIENTIFIC REPORTS 2017
Discovering selective diguanylate cyclase inhibitors: From PleD to discrimination of the active site of cyclic-di-GMP phosphodiesterases c-di-GMP signaling 2017
New insights emerging from recent investigations on Human Group II pyridoxal 5'-phosphate decarboxylases CURRENT MEDICINAL CHEMISTRY 2017
CHAPTER 4: Nitrite Reductase - Cytochrome cd1 RSC Metallobiology 2017
In silico discovery and in vitro validation of catechol-containing sulfonohydrazide compounds as potent inhibitors of the diguanylate cyclase PleD JOURNAL OF BACTERIOLOGY 2016
A pyrazolopyran derivative preferentially inhibits the activity of human cytosolic hydroxymethyltransferase and induces cell death in lung cancer cells ONCOTARGET 2016
How pyridoxal 5'-phosphate differentially regulates human cytosolic and mitochondrial serine hydroxymethyltransferase oligomeric state THE FEBS JOURNAL 2015
Screening and in vitro testing of antifolate inhibitors of human cytosolic serine hydroxymethyltransferase CHEMMEDCHEM 2015
Structural basis of functional diversification of the HD-GYP domain revealed by the pseudomonas aeruginosa PA4781 protein, which displays an unselective bimetallic binding site JOURNAL OF BACTERIOLOGY 2015

ERC

  • LS1
  • LS1_1
  • LS1_2
  • LS1_8

KET

  • Life-science technologies & biotechnologies

Interessi di ricerca

I started my academic career in organic synthesis and moved to Structural Biology in 2003, acquiring my PhD in 2006 in the Dep. of Biochemical Sciences. Since then I solved many protein structures using X-ray crystallography and have been mainly involved in the structure/function study of:

  • Human PLP dependent enzyme such as Serine hydroxymethyltransferase (SHMT), Dopa decarboxylase (DDC) and Alanine:glyoxylate amino trasferase (AGT) all involved in severe pathologies and/or cancer
  • Proteins from Pseudomonas aeruginosa involved in the regulation of biofilm formation or/and the denitrification pathway

As many others in the field I am now making the first steps in cryo-EM.

Finally, I’m also a freelance artist, specialized in: Cover Art; Web-Graphic Design; 3D modelling/animation and Scientific illustration (visit my website: giorgio-giardina.com)

Keywords

structural biology
PLP
PLP-dependent enzymes
protein conformational changes
x-ray crystallography
protein complex
Comunicazione scientifica
divulgazione scientifica

Progetti di Ricerca

Gruppi di ricerca

RNA-mediated modulation of cell metabolism

Gruppi di ricerca - Responsabile

Laboratorio di biochimica e analisi strutturale

© Università degli Studi di Roma "La Sapienza" - Piazzale Aldo Moro 5, 00185 Roma