Serena Rinaldo

Pubblicazioni

Titolo Pubblicato in Anno
Beyond the barrier: the immune-inspired pathways of tumor extravasation CELL COMMUNICATION AND SIGNALING 2024
Exploring the Metabolic Response of Pseudomonas putida to L-arginine ADVANCES IN EXPERIMENTAL MEDICINE AND BIOLOGY 2024
Molecular insights into RmcA-mediated c-di-GMP consumption: Linking redox potential to biofilm morphogenesis in Pseudomonas aeruginosa. MICROBIOLOGICAL RESEARCH 2023
Exploring Innovative Approaches to Isolate a One-Component c-di-GMP Transducer: A Pilot Study ADVANCES IN EXPERIMENTAL MEDICINE AND BIOLOGY 2023
The phosphodiesterase RmcA contributes to the adaptation of Pseudomonas putida to l-arginine FEMS MICROBIOLOGY LETTERS 2023
3-O-Methyltolcapone and its lipophilic analogues are potent inhibitors of transthyretin amyloidogenesis with high permeability and low toxicity INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 2023
A conserved scaffold with heterogeneous metal ion binding site: the multifaceted example of HD-GYP proteins COORDINATION CHEMISTRY REVIEWS 2022
Cytosolic localization and in vitro assembly of human de novo thymidylate synthesis complex THE FEBS JOURNAL 2022
Nutrient sensing and biofilm modulation: the example of L-arginine in Pseudomonas INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 2022
Riboregulation as a new player in the control of cellular metabolism: clues from the cryo-EM structure of serine hydroxymethyltransferase-RNA complex 2022
Inhibition of Glycolysis Impairs Retinoic Acid-Inducible Gene I-Mediated Antiviral Responses in Primary Human Dendritic Cells FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY 2022
Extracellular serine empowers epidermal proliferation and psoriasis-like symptoms SCIENCE ADVANCES 2022
Retinoic acid-induced 1 gene haploinsufficiency alters lipid metabolism and causes autophagy defects in Smith-Magenis syndrome CELL DEATH & DISEASE 2022
Studying ggdef domain in the act: Minimize conformational frustration to prevent artefacts LIFE 2021
High-fat diet leads to reduced protein o-glcnacylation and mitochondrial defects promoting the development of alzheimer’s disease signatures INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 2021
Modelling of SHMT1 riboregulation predicts dynamic changes of serine and glycine levels across cellular compartments COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL 2021
Importance of amino acids in brain parenchyma invasion by cancer cells ONCOSCIENCE 2021
Cytosolic localization and in vitro assembly of human de novo thymidylate synthesis complex 16th SIBBM Seminar Frontiers in Molecular Biology Frontiers in metabolic research Abstracts 2021
Signal transduction and c-di-GMP second messenger: novel tools to investigare the mechanism of nutrient sensing. WEBPRO - Proteins on the Web 2021. 2021
L- Arginine in Pseudomonas aeruginosa controls c-di-GMP levels and biofilm formation efb 2021 2021

ERC

  • LS1_1
  • LS1_2
  • LS1_7
  • LS1_9

KET

  • Life-science technologies & biotechnologies

Interessi di ricerca

The scientific activity of Serena Rinaldo is focused on the molecular aspects of proteins involved in biological processes crucial for cell maintenance and communication to dissect the mechanism of pathological conditions (including chronic infections and cancer), using a multidisciplinary approach encompassing Molecular Biology, Biochemistry and Structural Biology.

A schematic summary of the main research lines is reported below:

- Small molecules (nucleotides) involved in the formation of bacterial biofilm in chronic infections; integration of protein engineering and molecular biophysics approaches allowed the identification of structural determinants controlling the interaction of selected enzymes with small molecules (such as c-di-GMP, nucleotides and metals) and inhibitors, identified thanks to fruitful collaborations with medicinal chemists (Prof. Cappellacci l., Camerino, I). More recently, the role of strategic nutrients in controlling c-di-GMP levels has been investigated by mechanistic approach.

- Role of nitric oxide and redox metabolism in bacterial pathogens; the specific expertise acquired during the PhD and the first Post-Doc period of Serena Rinaldo allowed to re-evaluate the role of oxygen and the interaction of nitric oxide with metalloproteins and, broadly speaking, with the heme cofactor, as acknowledged by the publication records.

- Metabolic reprogramming in cancer cells and ageing, by looking at the role of enzymes of the serine/glycine pathway and one-carbon metabolism; she contributed to define the role of SHMT protein dynamic upon cofactor and substrates binding, relevant to rationally design compounds able to inhibit protein function. She developed a binding assay for testing enzyme inhibitors.

The main expertise is:

recombinant proteins production and characterization, protein/nucleic acid interaction, nucleotide and redox biochemistry, enzyme kinetics, microcalorimetry ITC, respiratory measurements (Seahorse).

Keywords

protein engineering
enzyme
cyclic nucleotide phosphodiesterase
energy metabolism
biophysics

Gruppi di ricerca

Gruppi di ricerca - Responsabile

Laboratori di ricerca

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