Ricerc@Sapienza

The JEOL JNM-ECZ 600R spectrometer is equipped with a 14.09 Tesla magnet, corresponding to the resonance frequency of ¹H of 600 MHz. For the specific needs of the metabolomic analysis, a 30-position autosampler which can be cooled down to -20°C and a cryoprobe was included in the supply contract.

The system is a liquid ultra-chromatography coupled with tandem mass spectrometry (UHPLC-MS / MS). Chromatography is equipped with a high-pressure binary pumping module and double-needle autosampler. The spectrometer is a triple quadrupole (QQQ) tandem mass analyzer with an ESI ion source.

Integrated Next-Generation Sequencing (NGS) system which consists of the following items:

The equipment is characterized by a Cell Imaging tool combined with a Multi-Mode Reader and an incubator-like system for real-time live cell and longer-term kinetic studies. The instrument should work by multiple advanced combined technologies, including fluorescence and high contrast brightfield imaging, monochromator-based ultraviolet and visible (UV-VIS) absorbance, high performance filter-based fluorescence and luminescence.

La tecnica analitica EBSD - Electron BackScattered Diffraction è dedicata all’analisi di materiali policristallini e monocristallini.

• L’apparecchiatura risulta attualmente l’unica in grado di processare un’elevata quantità di campioni, paragonabile a quella di una tradizionale realtime PCR. Questo sistema è l’unico che permette di lavorare in formato piastra da 96 pozzetti. La preparazione della piastra viene inoltre effettuata con modalità comparabili a quelle di un real-time PCR, rendendo immediata la transizione tra queste tecnologie.