Ricerc@Sapienza

  The ARP laboratory includes in detail the following equipment:

•  Applied Photophysics single-mixing -SX-18

•  Applied Photophysics Pi-star 180, 

•  Applied Photophysics sequential-mixing DX-17MV

• Tgk Scientific Hi-Tech PTJ-64 capacitor-discharge T-jump apparatus

Despite having an optimal sample for crystallization, obtaining crystals can be an arduous and unpredictable goal. The most important approaches to overcome this bottleneck were the discovery of appropriate precipitants, new methods of preparation of macromolecule samples, new methods of carrying out crystallization experiments but above all the increase of crystallization efficiency through automation.

The instrument is designed for high resolution mass spectrometry of proteins and nucleic acids. 

The source can be operated in positive and negative ion mode using the linear or reflector detector. The saturation free detector technology enables highest dynamic range and superior performance for high-mass detection.

A detailed description of each component of the Integrated Smart PHYTOTRON.

1) Walk-in chambers

The JEOL JNM-ECZ 600R spectrometer is equipped with a 14.09 Tesla magnet, corresponding to the resonance frequency of ¹H of 600 MHz. For the specific needs of the metabolomic analysis, a 30-position autosampler which can be cooled down to -20°C and a cryoprobe was included in the supply contract.

The Raman micro-spectrophotomiter is a Dilor instrument equipped with a red laser (632.5 nm), a Semrock edge filter and a 1800 grids/mm gratings. It is suitable for analysis of surfaces exploiting three objectives (10x 50x 100x).

FTIR-microscopy is an advanced technique that couples the capability of FTIR to provide a chemical analysis of samples, with the magnification power of an IR microscope. This combination allows to add a remarkable spatial resolution (e.g. <10 or <4 microns in reflection/transmission or ATR using Ge crystals) to the infrared spectroscopic analysis of materials, thus opening the door to spatial chemical discrimination of domains in inorganic or hybrid composites as well as in bio-materials.

Microscopio confocale spettrale ZEISS LSM 800 con 4 Laser su stativo rovesciato ZEISS Axio Observer 7 e sistema in super-risoluzione Airyscan per live-imaging e timelapse.

- Microscopio rovesciato da ricerca Axio Observer per LSM

- completamente motorizzato con passo 10nm (asse Z)

SEM da tavolo Hitachi 3000 per analisi in vuoto e semi-vuoto con sonda EDX per analisi semiquntitative