Ricerc@Sapienza

Il progetto proposto dal Dipartimento di Biologia Ambientale consiste in un Fitotrone “intelligente” integrato (Integrated Smart Phytotrone) che si propone di realizzare sperimentazioni interdisciplinari sulle reazioni delle piante a diversi tipi di stress: inquinamento, patogeni, siccità inter alia.

The Kaufman source acquired allows varying the proton beam energy from 10 to 1200 eV with a high degree of reproducibility and control. The same applies to the dose of incorporated protons. These very important parameters are typically hard to control by other systems, such as plasma sources or electrochemical methods. Indeed, Kaufman sources are configured to have the region of gas ionization physically separate from the intended target, avoiding exposure of the samples to intense and potentially damaging electric fields.

Spettrofotometro FTIR accessoriato per basse frequenze (Far-IR) e ATR

Despite having an optimal sample for crystallization, obtaining crystals can be an arduous and unpredictable goal. The most important approaches to overcome this bottleneck were the discovery of appropriate precipitants, new methods of preparation of macromolecule samples, new methods of carrying out crystallization experiments but above all the increase of crystallization efficiency through automation.

The instrument is designed for high resolution mass spectrometry of proteins and nucleic acids. 

The source can be operated in positive and negative ion mode using the linear or reflector detector. The saturation free detector technology enables highest dynamic range and superior performance for high-mass detection.

PESCA platform consists in the combination of AFM (with nm resolution) with an electrochemical set-up. This combination  affords simultaneous acquisition of different

types of images (topological activity of electrified surface and correlation with applied electrical potential) unveiling fundamental interfacial properties at nm range

A detailed description of each component of the Integrated Smart PHYTOTRON.

1) Walk-in chambers

SAXSLab Sapienza is a laboratory for structural characterization which operates the X-ray diffractometer Xeuss 2.0 HR P300K Q-Xoom (XENOCS), purchased thanks to the grant “Grandi Attrezzature d’Ateneo 2015”.

The instrument is capable of Small Angle X-Ray Scattering (SAXS), Wide Angle X-ray Scattering (WAXS) and Grazing Incidence Small Angle Scattering (GISAXS) experiments. These techniques are widely applicable for the characterization of matter at the nano- and meso-scale.

The JEOL JNM-ECZ 600R spectrometer is equipped with a 14.09 Tesla magnet, corresponding to the resonance frequency of ¹H of 600 MHz. For the specific needs of the metabolomic analysis, a 30-position autosampler which can be cooled down to -20°C and a cryoprobe was included in the supply contract.